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ncode human mirna microarray v3  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ncode human mirna microarray v3
    A <t>miRNA</t> <t>microarray</t> cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.
    Ncode Human Mirna Microarray V3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+human+mirna+microarray+v3/pmc03319589-109-8-13
    Average 86 stars, based on 1 article reviews
    ncode human mirna microarray v3 - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter"

    Article Title: HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034490

    A miRNA microarray cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.
    Figure Legend Snippet: A miRNA microarray cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.

    Techniques Used: Microarray, Expressing, Isolation, Stable Transfection, Transfection, Labeling, Plasmid Preparation

    Related Articles

    Ligation:

    Article Title: Circulating microRNA signature for the diagnosis of very high-risk prostate cancer.
    Article Snippet: Isolated serum miRNAs were added to a ligation mixture (200 U Truncated T4 RNA Ligase 2, 900 ng miRNA cloning linker II, 12% PEG 8000, and 1× T4 RNL2 buffer) from New England Biolabs following the manufacturer’s protocol and allowed to incubate for 3 h at 37 °C. .. The ligation mixture was suspended in 400 μL RNase-free 2× SSC hybridization buffer (0.3 M NaCl, 0.03 M sodium citrate, pH 7.0) and hybridized onto NCode Human miRNA microarray V3 (Invitrogen) for 12 h at 52 °C (29). ..

    Hybridization:

    Article Title: Circulating microRNA signature for the diagnosis of very high-risk prostate cancer.
    Article Snippet: Isolated serum miRNAs were added to a ligation mixture (200 U Truncated T4 RNA Ligase 2, 900 ng miRNA cloning linker II, 12% PEG 8000, and 1× T4 RNL2 buffer) from New England Biolabs following the manufacturer’s protocol and allowed to incubate for 3 h at 37 °C. .. The ligation mixture was suspended in 400 μL RNase-free 2× SSC hybridization buffer (0.3 M NaCl, 0.03 M sodium citrate, pH 7.0) and hybridized onto NCode Human miRNA microarray V3 (Invitrogen) for 12 h at 52 °C (29). ..

    Microarray:

    Article Title: Circulating microRNA signature for the diagnosis of very high-risk prostate cancer.
    Article Snippet: Isolated serum miRNAs were added to a ligation mixture (200 U Truncated T4 RNA Ligase 2, 900 ng miRNA cloning linker II, 12% PEG 8000, and 1× T4 RNL2 buffer) from New England Biolabs following the manufacturer’s protocol and allowed to incubate for 3 h at 37 °C. .. The ligation mixture was suspended in 400 μL RNase-free 2× SSC hybridization buffer (0.3 M NaCl, 0.03 M sodium citrate, pH 7.0) and hybridized onto NCode Human miRNA microarray V3 (Invitrogen) for 12 h at 52 °C (29). ..

    Article Title: Hsa-miRNA-765 as a Key Mediator for Inhibiting Growth, Migration and Invasion in Fulvestrant-Treated Prostate Cancer
    Article Snippet: .. For miRNA profiling, total RNAs were extracted and labeled directly using the NCode Rapid Labeling System (Invitrogen, Grand Island, NY) and arrayed on the NCode Human miRNA Microarray V3 (Invitrogen). .. Tissue expression of miRNA was studied by extracting total RNAs from cryosections (5–10 μm) using RNAzol RT (Molecular Research Center, Cincinnati, OH), poly(A)-tailed and reverse transcribed with universal RT primer using the NCode microRNA first-strand cDNA kit (Invitrogen).

    Article Title: Chronological Changes in MicroRNA Expression in the Developing Human Brain
    Article Snippet: .. We investigated 373 novel, putative miRNAs that are exclusively present on the NCode Human miRNA microarray V3 (Invitrogen), and found that 194 of these exhibited significant temporal expression changes. ..

    Article Title: HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter
    Article Snippet: After the miRNA was quantitated, poly (A) tail was added to the miRNA followed by ligation to a DNA polymer already labeled with Alexa Fluor dye using the NCode miRNA Rapid Labeling module (Invitrogen). .. The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation. .. The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation.

    Article Title: Hsa-miRNA-765 as a key mediator for inhibiting growth, migration and invasion in fulvestrant-treated prostate cancer.
    Article Snippet: .. MiRNA and gene expression For miRNA profiling, total RNAs were extracted and labeled directly using the NCode Rapid Labeling System (Invitrogen, Grand Island, NY) and arrayed on the NCode Human miRNA Microarray V3 (Invitrogen). .. Tissue expression of miRNA was studied by extracting total RNAs from cryosections (5–10 mm) using RNAzol RT (Molecular Research Center, Cincinnati, OH), poly(A)-tailed and reverse transcribed with universal RT primer using the NCode microRNA first-strand cDNA kit (Invitrogen).

    Article Title: MiR-193a-5p/ERBB2 act as concurrent chemoradiation therapy response indicator of esophageal squamous cell carcinoma
    Article Snippet: The isolated RNAs were labeled with dye at the 3′ position using the nCode Rapid miRNA Labeling System (Invitrogen). .. MiRNA arrays were generated on glass slides using nCode Human miRNA Microarray V3 (Invitrogen). ..

    Profiling:

    Article Title: Hsa-miRNA-765 as a Key Mediator for Inhibiting Growth, Migration and Invasion in Fulvestrant-Treated Prostate Cancer
    Article Snippet: .. For miRNA profiling, total RNAs were extracted and labeled directly using the NCode Rapid Labeling System (Invitrogen, Grand Island, NY) and arrayed on the NCode Human miRNA Microarray V3 (Invitrogen). .. Tissue expression of miRNA was studied by extracting total RNAs from cryosections (5–10 μm) using RNAzol RT (Molecular Research Center, Cincinnati, OH), poly(A)-tailed and reverse transcribed with universal RT primer using the NCode microRNA first-strand cDNA kit (Invitrogen).

    Labeling:

    Article Title: Hsa-miRNA-765 as a Key Mediator for Inhibiting Growth, Migration and Invasion in Fulvestrant-Treated Prostate Cancer
    Article Snippet: .. For miRNA profiling, total RNAs were extracted and labeled directly using the NCode Rapid Labeling System (Invitrogen, Grand Island, NY) and arrayed on the NCode Human miRNA Microarray V3 (Invitrogen). .. Tissue expression of miRNA was studied by extracting total RNAs from cryosections (5–10 μm) using RNAzol RT (Molecular Research Center, Cincinnati, OH), poly(A)-tailed and reverse transcribed with universal RT primer using the NCode microRNA first-strand cDNA kit (Invitrogen).

    Article Title: Hsa-miRNA-765 as a key mediator for inhibiting growth, migration and invasion in fulvestrant-treated prostate cancer.
    Article Snippet: .. MiRNA and gene expression For miRNA profiling, total RNAs were extracted and labeled directly using the NCode Rapid Labeling System (Invitrogen, Grand Island, NY) and arrayed on the NCode Human miRNA Microarray V3 (Invitrogen). .. Tissue expression of miRNA was studied by extracting total RNAs from cryosections (5–10 mm) using RNAzol RT (Molecular Research Center, Cincinnati, OH), poly(A)-tailed and reverse transcribed with universal RT primer using the NCode microRNA first-strand cDNA kit (Invitrogen).

    Expressing:

    Article Title: Chronological Changes in MicroRNA Expression in the Developing Human Brain
    Article Snippet: .. We investigated 373 novel, putative miRNAs that are exclusively present on the NCode Human miRNA microarray V3 (Invitrogen), and found that 194 of these exhibited significant temporal expression changes. ..

    Purification:

    Article Title: HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter
    Article Snippet: After the miRNA was quantitated, poly (A) tail was added to the miRNA followed by ligation to a DNA polymer already labeled with Alexa Fluor dye using the NCode miRNA Rapid Labeling module (Invitrogen). .. The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation. .. The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation.

    Sequencing:

    Article Title: HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter
    Article Snippet: After the miRNA was quantitated, poly (A) tail was added to the miRNA followed by ligation to a DNA polymer already labeled with Alexa Fluor dye using the NCode miRNA Rapid Labeling module (Invitrogen). .. The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation. .. The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation.

    other:

    Article Title: Autocrine/Paracrine Human Growth Hormone-stimulated MicroRNA 96-182-183 Cluster Promotes Epithelial-Mesenchymal Transition and Invasion in Breast Cancer
    Article Snippet: Basically, the miRNAs were extracted from MCF-7 MUT and MCF-7 hGH cells using the mirVana TM miRNA isolation kit (Ambion).

    Article Title: MicroRNA sensors based on gold nanoparticles
    Article Snippet: MicroRNAs (miRNAs) are small regulatory RNAs, the dysregulation of which has been associated with the progression of several human diseases, including cancer.. Interestingly, these molecules can be used as biomarkers for early disease diagnosis and can be found in a variety of body fluids and tissue samples.. However, their specific properties and very low concentrations make their detection rather challenging.

    Gene Expression:

    Article Title: Hsa-miRNA-765 as a key mediator for inhibiting growth, migration and invasion in fulvestrant-treated prostate cancer.
    Article Snippet: .. MiRNA and gene expression For miRNA profiling, total RNAs were extracted and labeled directly using the NCode Rapid Labeling System (Invitrogen, Grand Island, NY) and arrayed on the NCode Human miRNA Microarray V3 (Invitrogen). .. Tissue expression of miRNA was studied by extracting total RNAs from cryosections (5–10 mm) using RNAzol RT (Molecular Research Center, Cincinnati, OH), poly(A)-tailed and reverse transcribed with universal RT primer using the NCode microRNA first-strand cDNA kit (Invitrogen).

    Generated:

    Article Title: MiR-193a-5p/ERBB2 act as concurrent chemoradiation therapy response indicator of esophageal squamous cell carcinoma
    Article Snippet: The isolated RNAs were labeled with dye at the 3′ position using the nCode Rapid miRNA Labeling System (Invitrogen). .. MiRNA arrays were generated on glass slides using nCode Human miRNA Microarray V3 (Invitrogen). ..

    Glass:

    Article Title: MiR-193a-5p/ERBB2 act as concurrent chemoradiation therapy response indicator of esophageal squamous cell carcinoma
    Article Snippet: The isolated RNAs were labeled with dye at the 3′ position using the nCode Rapid miRNA Labeling System (Invitrogen). .. MiRNA arrays were generated on glass slides using nCode Human miRNA Microarray V3 (Invitrogen). ..



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    Image Search Results


    A miRNA microarray cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.

    Journal: PLoS ONE

    Article Title: HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter

    doi: 10.1371/journal.pone.0034490

    Figure Lengend Snippet: A miRNA microarray cluster was used to demonstrate the ability of HTLV-1 Tax to globally downregulate the expression of cellular miRNAs. The two lanes represent the Cy5/Cy3 ratio in dye flip. The miRNAs isolated from the stably integrated pooled Jurkat clone transfected with Tax were labeled with Cy5 whereas those isolated from the same integrated clone but transfected with control plasmid (pCMV4) were labeled with Cy3. The table shows the high probability miRNAs against P/CAF and p300 that have been downregulated in the presence of HTLV-1 Tax.

    Article Snippet: The purified tagged miRNA was hybridized to an NCode Human miRNA Microarray V3 (Invitrogen), which is an epoxy-coated glass slide printed with miRNA probes targeting all of the known human miRNAs in the miRBase Sequence database, release 10.0, in an antisense orientation.

    Techniques: Microarray, Expressing, Isolation, Stable Transfection, Transfection, Labeling, Plasmid Preparation